FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1975-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1975-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859653
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14370.16716047056195932No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA11090.12900554060766378No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG9980.1160933539463016No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9770.113650507821179No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC9690.11271989977351327No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT8820.1025995372551483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1200.066.4579541
TTACGTG1450.054.999692
TATACTG3500.051.7854235
CTCGACT450.00963889348.333068
AGCGAAA3000.045.916411
GTTATGT4300.045.5231
GTAATAT2500.043.499761
CCTATCC1451.9274012E-839.9997753
TTAGGTA2250.038.666454
GTTGACA6600.038.4467541
GCGAAAG3650.037.7395132
CTAGTAC800.002036512436.2497983
ACCGTGC1001.391333E-436.2497988
CGTGTTG2252.0008883E-1135.4442445
ACATGGT7500.033.8331455
GCTACTA1751.00895704E-733.1426739
CGAAAGC4200.032.7974323
TAGTCGG1551.464754E-632.7417538
TAGCATG13700.030.6932582
GCATGTT14050.029.9286584
Produced by FastQC (version 0.11.5)