FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1974-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1974-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020021
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1053.8204107E-641.434541
TATACTG5850.029.7449555
AGCGAAA4300.026.980631
GGTTCGA1907.155788E-626.7104458
GCGAAAG4850.025.413542
GTATATA5200.023.7053361
GGTTATA4002.7284841E-1123.5658931
CGAAAGC5350.023.0384433
CGGAGTA2552.7060905E-622.7461434
GGTAGCG2903.2862954E-722.4999318
TAGTCGG1952.6148476E-422.3076238
TACGTGT2303.1409865E-522.066233
GTTGACA7250.022.0031681
TTACGTG2003.102133E-421.7512
GTAATAT4701.0913936E-1121.5988541
GTAGTAC1700.003090253321.3266011
GTAGCGG2755.2109717E-621.0908459
GTTATGT6900.021.0175191
GAACCGA2104.3128748E-420.7152396
GCAGTGC8750.019.8885781
Produced by FastQC (version 0.11.5)