FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1974-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1974-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413467
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG6590.15938394116096327No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG6160.14898407853589285No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA6010.1453562194806357No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC5440.13157035507065862No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG4360.10544976987280726No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA4310.10424048352105487No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC4270.10327305443965297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG350.003565024862.1428579
GACGACT502.0088596E-457.9999924
GCTACTA651.0925169E-555.769239
GTTACGT901.3216759E-648.3333321
GTATAAG657.3315727E-444.6153831
TTACGTG1002.730234E-643.4999962
AAGACGA700.001055921541.428572
ACGACTC700.001055921541.428575
AGCGAAA1502.5931513E-838.6666641
CACTAGA1051.8518414E-434.5238085
CAGCTAC1102.4336914E-432.9545447
GTTATGT2900.032.51
TACGTGT1352.139183E-532.222223
CTACTGA5700.031.7982466
ACGTGTT1402.742813E-531.0714284
TATAATA2807.2759576E-1231.0714282
GGCATAA950.004724121730.5263181
CGTGTTG1453.4854966E-530.05
GTATTAT4650.029.6236571
CAGTGCA2701.70985E-1029.5370352