FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1973-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1973-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564148
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAGAGTGATCGCTTACACTGAAAAATATTTTGGCCTAAGTTTCATA20510.3635570807660401No Hit
GCTTTATACTGGGGCATTGTATGTTCGAGAAGCAATGTTCTATAAGAGGA9050.16041889716882804No Hit
TCTGTAGAGTGGGGATAATAATGGTAAACATTTAATTGGGTTGCTAGGTT8980.15917808801945588No Hit
ATTATGACCTGCGTGTACAATTTCTTAATGATAATTGTTGTCCACAAACC8870.1572282450704425No Hit
TCCCAGGACTCTGGGATCACAATCCAAGCTGAAGGCAGACCCTCAACCGA8850.1568737281706219No Hit
GTTTAATAAGATGGGGAGACTGGGACAGGAGCAGGTTGAGGAGGGATCAG8170.144820153576721No Hit
CTTAATAGACTGAGCCACCATGGTGTCCTAAAAAATTAATTTTCTAAAAT7560.13400738813219226No Hit
CCTTATAAGAGAGTTGGAACTCTTACCTGTGCCAAGGAATACTTCACCAC7560.13400738813219226No Hit
CTCTACCTCTGTCATTACATGCATATTTGCATGACAACAGACCATTTTTC6110.10830491289519771No Hit
TTATAAGAGAGTTGGAACTCTTACCTGTGCCAAGGAATACTTCACCACGT5850.10369619319752972No Hit
GGATAGAATTCATGGTGAAAATAAGACTCCTGTTACATATTCATGGAGAA5750.10192360869842665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG3300.0105.4545449
AGCGAAA3250.098.153851
GTGAGCG259.384611E-487.09
GAGTGAT4350.081.666677
GAAGCGC300.001935303772.56
GCGAAAG4700.070.957452
CTATACT851.6916601E-1068.23534
AGAGTGA5700.062.3245626
CTTCGCC350.003565697262.142857145
TCTATAC1400.062.1428573
CCGATAT606.8017634E-660.4166689
TCCACCG1700.059.705883145
TTAGAGT6900.055.6884044
AAGCGCC400.00604952454.3757
CCGCACT400.00604952454.3754
AGTGATC6800.053.3088268
TAGAGTG11450.053.1877755
CGAAAGC6350.052.5196843
CTCTATA2000.050.752
GACATAC901.3225399E-648.3333327
Produced by FastQC (version 0.11.5)