FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1973-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1973-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137674
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG15010.1319358621186737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1600.072.51
TTACGTG2250.051.5555572
GTGATCG1152.4847395E-950.4347849
GGTGCGA1252.719371E-740.6000028
AAGTCGA800.002036732936.251
CGTGTTG3850.033.8961035
GTAGCAT15100.032.6490061
CCGTCCG1402.7470052E-531.0714289
AGTCGAA1402.7470052E-531.0714282
TAGCATG13800.030.9963762
ACTTCGG950.00472756730.5263148
GTTATGT8100.030.4320981
TATACTG5750.030.260875
GCGAAAG4400.029.659092
GCATGTT14900.028.7080544
GTGCGAT1555.501078E-528.0645169
TACGTGT4150.027.9518073
AGCATGT15500.027.5967773
ACGTGTT4300.026.9767444
GTTGGCC16650.026.5615628
Produced by FastQC (version 0.11.5)