FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1972_lungMR_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1972_lungMR_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7571
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC160.21133271694624223No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC120.15849953770968167No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT110.14529124290054155No Hit
CCTGTGCGAAGAGACGGAAGCGGGGCTGCCAAGGAGACAGGAGGGGAGGA100.1320829480914014No Hit
CTTCTAGGAACAGGATGATTTTCTCACGCTGTAAGTGGGGAGACCAAGCA90.11887465328226125No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG90.11887465328226125No Hit
CTACTGTGACAGCCGCAACCTCCGGAAGGTCCCTGTCATCCCCCCACGCA90.11887465328226125No Hit
GGCAGGAACAGCAGCCCGTAGAACCGACTCTGATTTCCCAGTGAATGCTA80.10566635847312111No Hit
GTCCTGTGCCTCTGGTCCCAAGGGGCAGCAGCTCCTTGGCCACCTGTCCA80.10566635847312111No Hit
CCCTTGAAGTAGCCGTTCGGGATGGTCTCGATCCTGTTGCTGTCCAGGTA80.10566635847312111No Hit
GTCTTGGCACAAGAGTTGGCCAGGATGGGCTCCAGGGGGGCCGTGGGAGA80.10566635847312111No Hit
CCACAGAACTGGGGGCACAGTCTGAAAACAACTGAATTGTGGGATCTCTA80.10566635847312111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCAC100.0069530634145.06
TAATTTA100.0069530634145.04
TCCAGAG200.006001729.020-24
Produced by FastQC (version 0.11.5)