FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1972-lungUR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1972-lungUR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452626
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG4850.10715248350735486No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAGC350.003564047662.1481139
CACCGTA350.00356560862.141257
TTACGTG553.217527E-452.725912
ACCGTAG450.00963253548.337428
GCCACGA450.0096367448.332087
CTATATA1053.820149E-641.4274981
GTTACGT750.001482716438.6656651
TACGTGT850.00274075534.1167643
CCGTAGA950.004725189430.5255261
AGTGTAC950.004725189430.5255263
CTATAAC1000.0060717628.9992493
GTAACGG1000.0060717628.9992491
TAGTACT1306.478406E-427.8838944
GTTATGT3204.1836756E-1127.1867941
GCGAAAG1409.987623E-425.8921872
CGAAAGC1450.001225590824.9993533
CTTAATA2401.5952191E-624.166042
AGCGAAA1500.001493237624.1660391
GTTAATA1851.8288326E-423.5129051
CCGTCCT1550.001806077323.3890769
Produced by FastQC (version 0.11.5)