FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1972-lungUL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1972-lungUL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866697
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA13970.16118666615899213No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG13600.15691758480760865No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12640.14584104940942452No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA11730.13534141689656248No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA9660.11145763744422792No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT9270.1069577949387156No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC8780.10130414666255912No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC8670.10003496031485051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1350.069.814621
GTATCGG350.003566382762.1426851
TTACGTG1700.059.7091642
ATCGACA400.00605068154.37485145
GTTGACA7800.040.8973241
CGTGTTG2500.040.6022265
GCTACTA2900.039.999899
TACGTGT2450.038.47153
ACGTGTT2750.036.9111184
TAGTCGG2401.8189894E-1236.251998
TATACTG5050.035.893065
GATATAC1854.1782187E-935.272213
ACATGGT9200.034.675825
CCGTAGA1051.8535429E-434.5237121
GACATGG9700.032.8884054
CATTCTA10700.031.1699372
CTAGTAC1204.0562978E-430.2099933
CTACTGA13950.029.1055416
GTTATGT6500.028.9999181
CAGTGCA7650.028.4329342