FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1972-lungMR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1972-lungMR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779452
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA48210.2709261053402958No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG45100.25344881457887036No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG39640.22276521086267007No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA33260.18691147611736647No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT31760.1784819146568719No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT30100.16915319997392456No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA29900.16802925844585861No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA28520.16027406190220359No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG27390.153923792268631No Hit
GTCTGTCTGTCTCTCTCCCCAGCTCTGTGTCAAATAAATAAATGGCATCT26940.15139492383048264No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC25290.1421224062239386No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA23630.13279369154099127No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA22820.1282417283523242No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC22110.12425173592769011No Hit
AGTCAGTGGAGCTCTGTGACTCCTGATCTTGTGGTTTTGAGTTTCAACCC21560.12116089672550875No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC20710.11638414523122849No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT20700.1163279481548252No Hit
GCCCTGCACCAGGCTCCACACTCAGCGGGGATGCTGCTGGAGGATACCCT20220.11363048848746693No Hit
CATACAACCTACTAAGATTGAATTCTAAAGAAACAGACATCTGACTGTTC19530.10975289021563943No Hit
GATACTTGGGTGAGGATTTTTATGTTTATGTTCATCACAGATACTTGGGA19150.10761740131231413No Hit
CTTCCCGTATTGTAGAATTTGGTTTGCAGATATTTGGGTGAGGATTTTTA18770.10548191240898884No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCACAT18150.10199769367198441No Hit
GTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCATTAGTTTGAT18050.10143572290795143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT3450.081.9631961
TTACGTG3600.078.543652
TACGTGT4300.067.443563
CGTGTTG4900.059.185165
GTTATGT12750.058.004721
ACGTGTT5450.054.542664
GCTACTA4950.054.191769
GACGACT2250.051.556854
AGACGAC2550.048.334553
GGTGCGA1650.048.3331958
TGTGTTG14650.048.0046235
CAGTGCA13000.047.405042
TATGTGT16150.043.994923
TTATGTG17100.041.9747432
AAGACGA2800.041.4296152
CAGCTAC6550.040.954087
GTGCGAT1953.6379788E-1240.897329
AGTGCAG15650.040.304533
AGCTACT6700.040.0371978
TATACTG5350.039.3000535
Produced by FastQC (version 0.11.5)