FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1972-lungLR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1972-lungLR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844607
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11350.1343820261967992No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG10060.11910865053214098No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA9370.11093917052546333No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT8780.10395367312844908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1100.085.681821
TTACGTG1500.062.8333322
TACGTGT2350.040.1063843
CGTGTTG2450.038.4693875
GTTATGT5250.034.523811
ATATAGC900.00362791532.222223
GTTGACA7600.031.480261
GCTACTA1652.3858483E-630.7575769
ACGTGTT3151.8189894E-1229.9206354
GCTATAT1255.1531714E-429.01
GTTACGG200.00607664529.0110-114
GATATAC1753.7737009E-629.03
TGCGTTA1255.1531714E-429.08
TATACTG2252.8159775E-828.9999985
ACATGGT8300.027.9518075
GCGTTAA1358.081667E-426.8518529
CATTCTA7650.026.5359482
AGTCCGC2207.48365E-726.363638145
GGAACCG1100.0096707526.3636385
GTATTAT9150.026.1475411
Produced by FastQC (version 0.11.5)