FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1972-lungLL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1972-lungLL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949306
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3350.064.9287641
GCGAAAG4400.051.079522
CGAAAGC5350.042.009333
GTATATA9200.032.311471
ACCGTGC1050.007708271527.6190388
TTGTTCG1100.00967136126.3636257
ATCGTAT1409.994238E-425.8928478
TACCCGT1500.001494220324.1666586
ACGGATA2101.5549651E-524.1666588
GATTAGC1600.002174408422.6574341
GGGCGTT1600.00217507422.656247
CGTGATC1952.6146145E-422.3076868
CGGATAA2303.1418153E-522.065219
TCTACGT1650.00260076921.969693
TACACGT2053.666261E-421.2195034
GTCCTAT9000.020.9455411
TTCCGAC4501.3278623E-1020.9444374
ACACTGG13300.020.7142776
GCACCGT5950.019.495796
CCTATAC4102.1662345E-819.4512123
Produced by FastQC (version 0.11.5)