FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1971-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1971-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720566
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC8130.11282797134474842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT754.8530637E-967.666441
AGCGAAA2200.052.7270931
TTACGTG1004.7009962E-850.749832
GTGATCG1203.61797E-948.333179
GCGAAAG2900.042.4998552
GACGACT750.001483135238.666544
CGAAAGC3300.037.3483623
AGACGAC1204.0570108E-430.2082333
TATACTA1753.7727877E-628.9999055
TAACGTA1050.00770717227.6189545
CGTGTTG1907.151988E-626.7104365
TTAACGT1100.00966998626.3635464
TAGTGCA5100.025.588159
GTATTAT7450.025.3019311
GCTACTA1751.2552779E-424.8570619
ATTATAC2951.4266334E-824.5761893
GTTGACA6550.024.3510651
GTAGCGG1500.001493897624.1665869
TACGTGT2202.2277438E-523.0681043
GTCTAGA3152.8623617E-823.0157971
Produced by FastQC (version 0.11.5)