FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1970_d02_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1970_d02_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832137
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC9830.11812958683486013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3600.044.3107531
GGTACTG5650.037.2145165
GTACTGA5900.036.8665166
GCGAAAG4750.036.633672
ACTGATC6650.035.9773378
CGAAAGC4950.035.1535263
GCAGGTA6550.033.2080082
GGATAGC5100.031.2762995
ATTGTAC3306.184564E-1126.3651453
AGGTACT7700.026.3651434
ATTCGAC2507.818562E-826.1030621
CTGATCC9200.026.005369
TTGGACA6950.025.0374034
CAGGTAC8300.024.4592293
TAGGACG2151.8644232E-523.6065
TACCTCG1851.8303428E-423.5134477
GTACCGA1550.001808265523.387037
TTTGGAC7150.023.3230113
TATACTG3503.4979166E-922.7870185
TACTGAT8650.022.6299937
Produced by FastQC (version 0.11.5)