FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1970-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1970-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474493
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC10710.22571460485191563No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT6850.14436461654861082No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA5520.11633469829902654No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC4770.10052835342144141No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT300.00193351272.515131
TATACTG2100.058.690355
GTGATCG701.6946444E-551.7856039
GTTGACA4650.042.1055561
ACGAACC700.001056090341.4284825
ACATGGT4550.039.8350755
CCACGAA750.001482615738.666583
GTAATAG1157.124574E-637.8339771
CCTATAC1001.3903654E-436.2499243
TACTATA1854.1691237E-935.273912
TTATACT1656.0055754E-835.151444
TCTATAC6000.035.041593
CGAACCC850.002740569234.1175736
CACGAAC900.003626363432.222154
GCGTGCG1204.054915E-430.208279
GTACAGT1453.486622E-529.9999356
TTGACAT6200.029.2368892
GTGTAGG2502.4338078E-929.0060521
CTATACT1000.00607134828.9999374
CCGACTA1000.00607134828.9999374
Produced by FastQC (version 0.11.5)