FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1970-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1970-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843416
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG58660.20630115326072582No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT53560.18836498071333915No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG49490.17405121164120904No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC38160.13420477341338727No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT31730.1115911284173684No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA28710.10097010075205318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG18000.041.8908925
AAGGTCG9050.041.6572579
CGCTATA1252.7202805E-740.6019442
GACAGTA25950.039.1141787
AGCGAAA12000.038.667841
ACAGTAT26800.037.603098
GCGAAAG13500.037.0566751
GTGATCG4300.035.4068039
CAGTATG27950.034.758339
TTGGACA31000.033.4451524
GCAGGTA22450.032.9414442
CAGGTAC23150.032.2585643
GTACTGA25700.032.1610766
GTAGAAA31850.032.0972982
AGGTACT24750.031.637884
TATACTG24750.031.0519925
TTTGGAC35850.030.9429043
ATCCAAT36650.030.8598757
GTTACGT3600.030.209251
GTAATAT10600.028.7272851
Produced by FastQC (version 0.11.5)