FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1969_lungLR_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1969_lungLR_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894850
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC12780.1428172319383137No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG9880.11040956584902498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGC657.3331053E-444.6191642
GCTACTA1201.973549E-742.2905279
GTTACGT1252.7155147E-740.6057131
CGACCCG1105.2626383E-639.5466145
ATATCGG850.002739941234.1224481
TTACGTG1501.132963E-633.83622
GTTATGT4050.030.4363781
GACGATG2003.2528624E-729.00084145
ATATACC1555.4987013E-528.0653294
GTGCGAA1555.4987013E-528.0653294
CCGACTA2603.7180143E-927.885424
CTATATA2354.2906322E-827.7698651
GTATAAT1606.8208115E-527.1913241
AGGTCGG4650.026.506148
GCTATAT1100.00966442426.3673441
GTATTAT7250.026.0036581
TATACTG2808.190909E-925.8936065
AGCGAAA5800.025.0035171
TACGTGT2101.554483E-524.1673643
GTTGACA8800.023.0714261
Produced by FastQC (version 0.11.5)