FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1969-lungUL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1969-lungUL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344585
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3950.038.5441671
CGACCCG1157.1447193E-637.82595145
GCGAAAG4300.035.406852
TATACTG8850.035.2258645
ATCGTGC1402.747441E-531.0713188
CGAAAGC5200.030.6729683
TGTACGG1000.006075298428.9998972
GTCTACG1306.484256E-427.8845181
TGACGCG1050.00770951727.618948145
ATACTGT13050.024.999916
GTTGACA9300.024.166581
GTGATCG1851.8311676E-423.513439
TATCGTG1902.1937677E-422.8946577
GTAATAT7800.022.3076111
TACTGTG14800.022.043847
TAGTACT3004.557496E-721.7499224
GGGATAC7850.021.241962145
GGTCCTA16600.020.9637819
TTAGAGT7750.020.580574
ACATGGT11050.020.3392945
Produced by FastQC (version 0.11.5)