FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1969-lungMR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1969-lungMR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655228
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCG100.0070887697145.010534
AGCGAAA2000.050.757561
GCGAAAG2350.043.1946262
GTGATCG1102.4352898E-432.9544269
CGAAAGC3150.032.224563
CCTATAC2003.24937E-729.0021063
TATACTG3408.913048E-1125.5900945
TACTAGT1801.5187568E-424.1684232
GTATAAG2353.7024096E-521.5989631
CTATACA3757.743438E-921.2682114
TCCTATA3951.4084435E-820.191342
GTATTAG2205.8944646E-419.7756731
ATAGGTG2959.547979E-619.6624475
TCTATAC7600.019.0803323
CTATACT3051.27304975E-519.0177764
TACTATA3051.27304975E-519.0177762
CCTATAT3852.380093E-718.8325373
TTATACT4303.7320206E-818.547864
GATATAC2359.186258E-418.5133951
TTATAGC3201.9250792E-518.1263164
Produced by FastQC (version 0.11.5)