FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1969-lungLR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1969-lungLR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19822
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA370.18666128544041974No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG290.14630208858843707No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA270.13621228937544144No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT210.10594289173645444No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT210.10594289173645444No Hit
GTAATATACTGTGCTGCTTCTGAGAAACCAGCAGGGCCTGTTAATTCTTT200.1008979921299566No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC200.1008979921299566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACTC100.007038828145.00002145
TCCTCGC100.007038828145.000027
CCTCGCC100.007038828145.000028
GTGTATG100.007038828145.000023
CTCGCCT100.007038828145.000029
TATACTG100.007038828145.000025
TTCCTCG100.007038828145.000026
Produced by FastQC (version 0.11.5)