FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1969-lungLL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1969-lungLL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235475
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC5650.033.365153
GCGAAAG7150.026.3654692
AGCGAAA7450.026.2780571
GCATCGA3902.0008883E-1124.166391145
ATAGCGC3102.4194378E-823.3868279
TGTAGGG7250.021.001462
GTTACGT1750.00365687920.7165641
GATAGCG3508.7413355E-820.714058
TACACTA3101.4663552E-518.7109785
CTACACT6251.8189894E-1218.561294
GATGCGC5901.2732926E-1118.4319928
GGATAGC5158.039933E-1018.3007627
GTCCTAT9400.017.7413121
GTGTAGG7800.017.66221
ATACTAT5351.3351382E-917.6180486
GTATAGT2901.864251E-417.5019261
TAGGACC8600.016.8616374
GTATTAT12550.016.7548311
TATAGTG8500.016.2070085
ATAAGAC5402.8539944E-816.112233
Produced by FastQC (version 0.11.5)