FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1968_lungLR_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1968_lungLR_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843668
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA12790.15159991845133394No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG12190.14448811617840193No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11940.14152486523134694No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT10500.1244565397763101No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT10280.12184887894290172No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA10030.1188856279958467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1800.060.4166681
TTACGTG1900.057.2368432
GTGATCG901.3233275E-648.3333329
TACGTGT2350.046.2765963
TAGTCGG1950.044.6153878
CGTGTTG2500.043.55
TATACTG3850.039.5454565
GCTATAT1157.1413833E-637.8260841
CAGCGCG800.002036435336.259
GATATAC1604.588219E-836.253
ACGTGTT3050.035.6557354
GACGACT1153.1616844E-431.5217364
GTTGACA9550.030.3664931
AGACGAC1204.0575134E-430.2083343
GTAATAT3151.8189894E-1229.9206351
CTACTGA10950.029.132426
TAACCTA3001.8189894E-1129.0000025
GTTATGT6750.029.0000021
GCGAAAG2003.253317E-729.02
GTTAATA3800.028.6184221
Produced by FastQC (version 0.11.5)