FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1968-lungUR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1968-lungUR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743578
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3450.039.92931
GCGAAAG3700.039.1909182
GTATAGC1551.4638335E-632.7433781
ATGCGTT1352.1418075E-532.221474145
CGAAAGC4650.031.184173
GTACCAC1902.0745756E-730.527666
TACGACA1000.006072160329.0012782
GCTATAC1306.4797036E-427.8858453
ATACCGT1409.989603E-425.8946
TAATATA2301.1020547E-625.2185044
CTATACC4151.8189894E-1224.4589084
AGATTGT3702.6011548E-1023.514556
TCTATAC3102.4147084E-823.3881283
GTAAGAT5650.023.0983623
CTAATAC3803.619789E-1022.8957443
TATATAG2552.704499E-622.7461015
GTATTAG1952.6130912E-422.3086741
TATATAC2404.3594682E-521.1467653
TAGATTG5600.020.7151995
TATAGCA3208.461102E-720.3915232
Produced by FastQC (version 0.11.5)