FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1968-lungUL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1968-lungUL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1438214
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA21480.1493519045149053No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG20430.14205118292548954No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG19760.13739262724462423No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT14810.10297493975166422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2250.051.562391
TTACGTG2700.042.9686622
GATATAC2401.8189894E-1236.2535483
GCTACTA3750.034.8009839
TATACTG5050.034.4588175
GTTATGT9550.034.1668321
CGGACGC1102.4367703E-432.954334145
AGTCCGC2807.2759576E-1231.071228145
CACCGTT950.00472812630.526127
GTTGACA15750.030.3849811
AGCGAAA4950.029.2968141
CGCATAA1000.00607552328.999813145
CAGTGCA9850.028.709392
ACATGGT15800.028.452155
TAGTCGG2354.2991815E-827.766748
GGTTCGA2903.965397E-1027.5007788
GTTCGAT2954.820322E-1027.0346629
CGTGTTG4350.026.6692755
GCGATTA1100.00967327226.363466145
GGATATA3357.4578566E-1125.9735952
Produced by FastQC (version 0.11.5)