FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1968-lungMR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1968-lungMR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699253
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10260.1467280083174473No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG9260.13242703284791055No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA8790.12570557437722826No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT7870.11254867694525444No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG7030.10053585755084354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA5250.045.5840151
TATACTG2600.044.624525
GTTACGT1002.7277674E-643.5120161
GCTACTA1505.366019E-1043.4995739
ACATGGT5800.042.50875
TTACGTG1157.1261093E-637.8365332
GTTATGT4800.037.770851
CGGCGTA1604.5780325E-836.257423
TACGTGT1301.6517562E-533.468393
TCGGCGT1801.2883174E-732.2311252
CTCGGCG1902.0700645E-730.5347481
CGTAAGG950.00471995230.5347482
GGCGTAG1902.071356E-730.5325624
GAGCGAA1204.0502497E-430.2166791
CAGTGCA4950.029.3010222
ATATAAC3001.8189894E-1129.008012
TATGTGT5800.028.7558843
TATAACC2802.6193447E-1028.4879723
TATTGGG4600.028.3774012
GACATGG8250.028.1269684
Produced by FastQC (version 0.11.5)