FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1968-lungLR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1968-lungLR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083488
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC16410.15145529992025755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGCG553.218733E-452.728565145
CCGGTAA750.001483262438.667614145
TATACTG4400.031.306145
ATATACG1000.00607532128.999374
GTTGACA9800.028.1129341
TTGTCGT1050.007709545527.618459
GTTCGAT1409.996395E-425.8922969
AGCGAAA4750.022.8952981
ATATAGC2252.6522499E-522.5550673
ACATGGT12100.022.1689415
GTATAGG2953.8740836E-722.1191861
TATAATA8550.022.0463072
GTATGAC3655.68798E-921.8488433
GGTCCTA16000.021.7495279
GTAATAT2704.4444805E-621.4820081
GAATAAC5800.021.250521
CCATCGA2404.3640444E-521.1453749
ATACAGC9000.020.943996
GTATTAT9100.020.7147941
TATATAC4952.1827873E-1120.5046063
Produced by FastQC (version 0.11.5)