FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1968-lungLL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1968-lungLL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821668
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG8420.10247447874323935No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG8400.10223107143031006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCGA606.8045174E-660.4166688
GTTACGT1109.9564204E-846.136361
AGTCGAA803.7340687E-545.31252
CGACCCG700.00105653141.42857145
AAGTCGA907.481275E-540.277781
CCGCGTC750.001483231938.666668145
TATACTG2650.038.3018885
CGGAGCG1354.969952E-737.592594145
GCTACTA1803.1977834E-936.259
GTTGACA6500.035.6923071
GTGCGAT1251.2648135E-534.89
GTCGAAG1051.8534291E-434.5238083
ACATGGT7150.033.461545
AGCGAAA3050.033.278691
CCGACTA2404.3655746E-1133.2291684
TATACTA1102.4357735E-432.9545445
GGTTCGA1204.0574223E-430.2083348
GTATATC1703.010853E-629.8529421
ATAATGC1000.006073834429.03
TTACGTG1804.6986243E-628.1944452
Produced by FastQC (version 0.11.5)