FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1968-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1968-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661264
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT52910.8001342882721576No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG49640.7506835394033246No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG49400.7470541266423093No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT37620.5689104502891432No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA36810.5566611822207167No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT36380.550158484357231No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA34210.5173425439763847No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG30110.45534007597570714No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC26150.39545476541895525No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC25720.3889520675554695No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA23820.36021921653076533No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG23200.35084323356480923No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA20660.312431948510731No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC20640.31212949744731305No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA20110.3041145442667377No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC20060.3033584166081928No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT18300.27674272302741415No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG17840.26978634856880157No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG16530.24997580391492658No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT15290.23122383798301432No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT14660.22169662948534927No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC14370.21731108906578914No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC13920.21050594013888552No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA13880.20990103801204965No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA12420.1878221103825401No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT12300.18600740400203247No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG12300.18600740400203247No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT12070.18252921677272618No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG11920.18026083379709162No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA11790.17829490188487504No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT11700.1769338720994943No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG11400.1723971061482252No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT10940.16544073168961262No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA10910.16498705509448572No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG10840.16392847637252292No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC10840.16392847637252292No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA10670.16135764233347044No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC10600.16029906361150764No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG10590.16014783807979868No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC10390.1571233274456193No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTC10050.15198165936751434No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT9980.15092308064555154No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACT9410.1423032253381403No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT9330.14109342108446854No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT9220.13942994023566987No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA8980.1358005274746546No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT8960.13549807641123668No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT8890.13443949768927388No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT8810.13322969343560212No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA8760.13247356577705727No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG8700.13156621258680345No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA8660.1309613104599676No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC8660.1309613104599676No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG8520.12884415301604202No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC8480.12823925088920612No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA8460.1279367998257882No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT8460.1279367998257882No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT8370.12657577004040746No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG8350.1262733189769895No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA8190.12385371046964601No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT8070.12203900408913838No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA8060.12188777855742941No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG7990.12082919983546662No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG7980.12067797430375767No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA7900.11946817005008589No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC7790.11780468920128723No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC7720.11674611047932444No Hit
GCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAGGCGAATCAATA7650.11568753175736166No Hit
GATACTACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCT7520.11372159984514507No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA7490.11326792325001814No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT7380.11160444240121947No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA7350.11115076580609257No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG7350.11115076580609257No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT7260.10978973602071185No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA7250.10963851048900287No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC7250.10963851048900287No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG7110.10752135304507732No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG7050.1066139998548235No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA6990.10570664666456966No Hit
ATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACAC6970.10540419560115173No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT6830.10328703815722616No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG6740.10192600837184544No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG6690.10116988071330059No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC6660.10071620411817368No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGC100.007090344144.999859
GCAGGTA6150.0111.991752
GGTACTG6350.0108.464455
AGTAGAA8500.0107.486741
GTACTGA7450.094.3958746
CAGGTAC7500.091.833233
ACTTAAT259.38537E-486.999912
AACACTG4600.078.804265
GCGAAAG1900.076.3157042
TAATCCA8000.074.312425
AAATATA3550.073.521055
TTTGGAC10750.073.511553
GTCATAT502.303319E-672.510891
GGTCAAA3500.072.510891
ATATCAC502.305389E-672.499923
AGGTACT10300.071.0921554
AGCAGGT10050.069.264131
GCTTTAA8500.069.098621
CTGATCC10600.069.080129
ACTGATC11450.068.384218
Produced by FastQC (version 0.11.5)