FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1967_d08_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1967_d08_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878266
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3400.049.046891
GCGAAAG4150.040.1807062
GTTACGT1051.8529166E-434.525761
GTGATCG1051.8535367E-434.52389
CGAAAGC5250.031.761893
GTAGCCG1204.0576578E-430.208329
GGTAGCG2405.270158E-827.1874898
TAGTCGG1907.154158E-626.7105168
GTAGCGG2559.483483E-825.5882269
TTACGTG1450.001226327424.999992
GCTACTA2901.1901648E-824.999999
TATACTG4103.8198777E-1122.9877955
GTTATGT6950.022.9509371
AGGTCGG3503.4997356E-922.7857068
GTGCATA2953.871155E-722.1198941
GTCTTAT3304.6855348E-821.9709381
CTACATC5051.8189894E-1221.5346453
GTCTAAG4409.822543E-1121.4216651
ATAATAC11250.021.2666573
GCACCGT6200.021.0483786
Produced by FastQC (version 0.11.5)