FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1967_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1967_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286645
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG1450.060.0100362
AGCGAAA1400.056.9738121
CGAAAGC1850.047.034893
GTCCTAT1604.5632987E-836.2560651
GTATGGT1209.529123E-636.2560653
GTCTTAT850.00273649334.1233521
GGCATAC850.002738835534.1173977
CTATAAG1352.1344624E-532.227611
CTAGATA950.004717867430.531424
TACGTGT950.004717867430.531423
ACCGTGC1000.006067541428.999798
ACACCGT1306.465256E-427.889286
ACCTAAT1100.00965242426.3680461
GTATAAT1100.00965242426.3680461
TGTATGG1409.967419E-425.8971882
ATTACAC2101.5480377E-524.170713
TCCTATG2151.8573148E-523.6085992
ATAGGGG1550.001803600823.3910083
CATACTC1550.001805433223.3869299
TAATACA2303.1279036E-522.0689094
Produced by FastQC (version 0.11.5)