FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1967_d04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1967_d04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585629
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGGACTGTGCAGTTTAAGGCAGTTAACTCTACCTTGAAACCCAAAG7940.13558071748496062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1500.077.333331
GTATAGG702.8103386E-972.51
GCGAAAG2200.056.022732
GGACCGT651.09308185E-555.769236
GAGCGAA701.6951151E-551.7857131
CGAAAGC2400.051.3541683
TTATACT1652.7284841E-1143.9393964
TATAGGC1002.732193E-643.52
GCAGCGT1105.260563E-639.5454568
GTAACCG750.00148288438.6666643
TATACTG2500.037.75
GTACAAG1458.69024E-735.01
CTGTGCG1352.1406957E-532.222229
GAACCCC3000.031.4166666
AACCCCG2558.731149E-1131.274517
AGCTATC2351.2605597E-930.8510657
ATGATCG950.004725713330.5263185
GATCGTG950.004725713330.5263187
GACCCAT3100.030.4032277
CTATAGC2202.260822E-829.6590923
Produced by FastQC (version 0.11.5)