FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1967-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1967-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584997
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTATCCTATGGGGAATTAATGGAAGATCCAGAACTAGAGCCTAGGTT8870.15162470918654283No Hit
GCCTAAGGGAGGATGACCAAGAAGCATGGGGCTGCGGTCTTTGGTATCAA8450.14444518518898386No Hit
ATCTAGTCCCTATCCCTGAACTCAGCCCAGAGCCGCCTTCTGCACATCTG8430.14410330309386202No Hit
CAATATACAGATTACCTTTTTTTAAACTGGAGTTACCCAACAAGTGGACC7940.13572719176337655No Hit
TCTATGGGCAGTGTATTTTTTGCTACCCCAAGCTGCCTGCAGGCTTTCTG7590.1297442550987441No Hit
TCACTAGACAGTGGCTATGGAGAAAAGTAGATGGAATCAAAAAATACTGA7570.12940237300362226No Hit
TGGTAGAGCAGGTGTTGAGGCACAAGGACACCAGCCAAGGGAGCAAACGA7300.12478696471947719No Hit
GTGCAGTGTGGTCAGTGACGCAGCACTCCGGGGAGAGATGGAGACGCAGA5850.10000051282314268No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC151.2294952E-4145.00743145
AGCGAAA1900.087.7601551
GCGAAAG2200.075.792862
GCCTAAG3600.050.34551
ACGGGAC450.00963845448.331688
CGAAAGC3700.045.066033
GCTAGGG1105.261789E-639.54413
CTTACGG750.001483131938.6653443
TATCCTA2500.037.698715
ATAGTGG2650.035.564823
GGGTCGG2700.034.9062169
GTAGGCC850.002741519634.1164789
GGGGTCG2850.033.0690468
CTAAGGG5500.032.953423
TCTATAC4700.032.392513
CCTTTAG2257.912604E-1032.223877145
CTAGACG900.003627617132.221129
ATGACGT1153.1611408E-431.5206629
ATCCTAT3050.030.9005856
GGCTTAC950.004726498430.5252721
Produced by FastQC (version 0.11.5)