FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1967-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1967-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933897
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAGACTGCTCAGGGTATAGCCCAAGGAACGGGGCTAAAGATAGAAG19330.2069821404287625No Hit
ACTTATTACCGTCTTCAGAAAATCCAAGATATGGAAGGTGGAAGAAAGAA13580.14541218142900125No Hit
GTATATACTCAATAGGTAAGCTGAATGCTTACCTGCTTTATAAAGTCTGT11340.1214266669664856No Hit
GTCACACACCCTGTGGGAATAAGTCACAGTTTCCCAAGTGTTCACTACAG10140.10857728421870935No Hit
ATATGGAACAGCTGGGGTAGTAAACTTTAGTAGCAGTATTCAACTTTGGC9440.1010818109491732No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2800.093.238461
CGAAAGC3000.091.842393
GCGAAAG4050.066.2446442
ATACCGT451.1932346E-464.45086
GCGTACT502.0102593E-457.999517
TTACCGT3700.052.910626
AGCGTAC553.2173257E-452.732476
ATTACCG4000.048.942335
ACCGTCT4150.047.168278
CCGTCTT4350.044.999629
TACCGTC4850.044.844987
TATTACC5150.043.6450774
GCTTACA1703.8198777E-1142.6535452
ATAGGCG700.001056092841.4326555
CGTACTA700.001056649441.428228
GGCTTAC2401.8189894E-1236.25941
AGACTGC8850.032.771596
TACCGTG900.003628259832.221957
TATAGTA2755.456968E-1231.6411762
ATGGAAC4500.030.6141283
Produced by FastQC (version 0.11.5)