FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1967-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1967-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966164
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC15390.15928972720987328No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12570.13010213586927272No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT9790.1013285529164821No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG400.00604965354.377665
GTTACGT1501.0913936E-1148.33821
TTACGTG2005.456968E-1239.879012
GTTGACA9500.029.0029221
CCTTACG1255.1506783E-429.0029162
GCTACTA2603.7198333E-927.8845359
GTATAAT3408.913048E-1125.590811
GGTTATA3201.2387318E-924.9243851
GGTCCTA13150.024.8098149
ACGTGTT2951.4269972E-824.577474
TACGTGT3001.7060302E-824.1678493
TACACGG2101.554409E-524.1678475
ACATGGT11200.023.304715
CCTTCGC1600.00217513322.6561859
TTGACAT11850.022.639412
AGCGAAA3955.8935257E-1022.0275341
GTTATAT3304.6844434E-821.9719092
TTACACG1650.002600058421.9707724
CAGTGCA8650.021.79412
GTATATC2003.1008225E-421.7521881
Produced by FastQC (version 0.11.5)