FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1967-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1967-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864369
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12170.1407963497071274No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA9480.10967538169462349No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA9290.10747724640749495No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC9080.10504772845856342No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG9010.10423788914225289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2100.041.428341
AGCGAAA2800.038.8390661
GCGAAAG3150.034.5256162
TTACGTG2603.6379788E-1233.4632872
CGAAAGC3300.032.956273
GCTACTA2206.220944E-1032.954369
TACCGAT950.00472573630.527917
TACGTGT2752.1282176E-1029.0015163
ATACCGA1358.079353E-426.8532566
ACACCTA10100.026.5592581
TCGATTA7400.026.452557145
GTAGCAT12300.025.9348141
GTGATCG1409.993819E-425.8927129
TACTGAA13200.025.8157427
GTATGAC2559.478208E-825.5895753
CTACTGA13950.025.4672816
GTTATGT7500.025.1331921
CACCTAA11350.024.9131972
TATACTG4750.024.422335
ACCGTGC1550.001807741823.3883198
Produced by FastQC (version 0.11.5)