FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1966_lungUL_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1966_lungUL_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG50.40192926045016075No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA50.40192926045016075No Hit
GATACTTGGGTGAGGATTTTTATGTTTATGTTCATCACAGATACTTGGGA40.3215434083601286No Hit
CCCCATGCTGACCAGGGACCTCATGCAGGGCTCAATTCCAGGACCCTAAA40.3215434083601286No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA40.3215434083601286No Hit
CAAATAAGCAACCTAACTTGATACCTCAAGGAAACAGAAAAGAACAAATA30.2411575562700965No Hit
ACACCAACAAGAATAACAGATCAACAAAGAGCTGGTTCTTTGAAAAGAAC30.2411575562700965No Hit
CCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAGAA30.2411575562700965No Hit
AGTATATAGTGATACAGGCCTAACTCAAGAAATAAGAAACATCTCAAAGA30.2411575562700965No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT30.2411575562700965No Hit
AATGACATCTTTAAAAAATAAAGTGAGCTCTATGCCCAGTGTGGGGTTGA30.2411575562700965No Hit
ACTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGC30.2411575562700965No Hit
CCCACGAACCCAATTGGTTGTTCAGTAGAATGTTGTTTAGTCTCTGTGTT30.2411575562700965No Hit
GGCCTGTATCACTATAAACTTCCTTCTTAGAACAACTTTTTTTTTTTTTT30.2411575562700965No Hit
CTTCCCTCAATGATCCCCCATCCAGACAGAAGATCACAAAGGAAACATCA30.2411575562700965No Hit
GGCCTGTCCTGTGCCTCTGGTCCCAAGGGGCAGCAGCTCCTTGGCCACCT30.2411575562700965No Hit
CAGAAATACTTTGAGGTAAATGAAAAAATAATACAATGAAACAAAATTCA20.1607717041800643No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA20.1607717041800643No Hit
GTATCTCCTAGTTTATAAAGCACTTTTATACACATCCTCGTATTGGAGTC20.1607717041800643No Hit
GCTTAAATAAGAAATCAAAAACTTCCAACAAGCAAAAGTCCAGGACCCCA20.1607717041800643No Hit
GTTTACTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA20.1607717041800643No Hit
CCTGCCAGGATTGAATCCTAAAGAAACAGAAATATGAACAGACCAATGAG20.1607717041800643No Hit
GTAGCATTTGGTTTGCAGATACTTGGGTGAGGATTTTTATGTTTATGTTC20.1607717041800643No Hit
AGTGGAAGGAGCAGCAGGCAGTAGAAGAGAGAGAAAGGGGCTCCCCGTGC20.1607717041800643No Hit
CCTATAATTTTCCCAAATGAGGACTGAAATCAAATCAACCATCTTTTCCA20.1607717041800643No Hit
ATGGCACAGGGTGTGGGGACCAGCCAGGCGGCAGAAGCACAGGTCGGGGT20.1607717041800643No Hit
TTCTGCATCTGTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAA20.1607717041800643No Hit
GATATGTTCCTTCTATTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCC20.1607717041800643No Hit
CTCATGCAGGGCTCAATTCCAGGACCCTAAATTCATGACTTGAGCCAAAG20.1607717041800643No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG20.1607717041800643No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA20.1607717041800643No Hit
GACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCAAAAGGAAT20.1607717041800643No Hit
ACATTCTACTGAACAACCAATTGGGTTCATGGGCAAACCAAAATAGGAAC20.1607717041800643No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC20.1607717041800643No Hit
GGATATGGTCATTGGAGAAGAATTTATTGTGTTTATTTACAGAGGAAAAA20.1607717041800643No Hit
CTTAATAAGCAACCTCAAGGAAACAGAAAAGAACAAATAAAGTCAAAAGT20.1607717041800643No Hit
CCTCTCTACACAGCAGATTTAAAAATACAGAGTGCTAAGGTTGGCACAGG20.1607717041800643No Hit
GCTGGAGAGAGAGACAAACTGACAGACTGAGCAAAGTGGAAGGAGCAGCA20.1607717041800643No Hit
GTTCCAGGGGCACTTGAGAGGAGTATATTCCTTTTGCTGCTTTTGGATGG20.1607717041800643No Hit
AAATACATCTTACTGGATCATACCTCTTCCCGTATTGTAGCATTTGGTTT20.1607717041800643No Hit
CAGATTCTCAGCGAGATTCAAAGAAAGAGGACTCATCATCAGAAATGAGA20.1607717041800643No Hit
GTACCCTTTTCTTTTGGATTATCCAGCTTGTTGGGGTGTAATTGTTCATA20.1607717041800643No Hit
AGTCTGTTCATATGTCTTTTTCTTTAGAATTCAATCTTAGTAGGTTGTAT20.1607717041800643No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA20.1607717041800643No Hit
GAGCAGGGGTGTGCTTTGTTCATGGCTGCAGAACTAACAGATGATCTTCT20.1607717041800643No Hit
CTCCCGCTCTGTGTCAAATCAATAAATGACATCTTTAAAAAATAAAGTGA20.1607717041800643No Hit
CACATCCTCGTATTGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTG20.1607717041800643No Hit
GAGGCTGATGTTTCCGTTGTGATCTTCTGTCTGGATGGGGGATCATTGAG20.1607717041800643No Hit
TTCTACATCTGTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAA20.1607717041800643No Hit
CCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAAA20.1607717041800643No Hit
CCCAAGACGGGAGCGTGTCTACAGCATTTTCCGTTCTTTCCAGGACCTCC20.1607717041800643No Hit
GAATCTCTTCTATTTAAATTTAAAAATAGTTTTTAGAATTGAGTGAAATG20.1607717041800643No Hit
AGGATATACTGCCCAATTATGAAAATGATTGCACAACCACCAAATGTTCA20.1607717041800643No Hit
AGTTTATAGTGATACAGGCCTAACTCAAGAAACAAGAAACGTCTCAAATA20.1607717041800643No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA20.1607717041800643No Hit
ATTTTACTGCATTAATTCCATTATCAGTTGTAGTGCCCTTCATTGGCTCT20.1607717041800643No Hit
GTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACATTAGTTTGAT20.1607717041800643No Hit
CTCTCCCCAGCTCTGTGTCAAATAAATAAATGGCATCTTTGAAAAAATAA20.1607717041800643No Hit
AGTTAGTAGAAGGTACACATCAGAACTTAAATAAAGTTCATCATCACTAG20.1607717041800643No Hit
GAAGCACAGGTCGGGGTGGCAGTGATTTGGTAGCAGCGCTATCAAATGCT20.1607717041800643No Hit
ATACCACAGGGAACTTTAATACCTCACACTTCTCTCAATGATCCCCCATC20.1607717041800643No Hit
GTTTCCTTTGTGATCTTCTGTCTGGATGGGGGATCATTGAGGGAAGTGTG20.1607717041800643No Hit
CCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCATTAGTTTGATTT20.1607717041800643No Hit
GCCCTGCACCAGGCTCCACACTCAGCGGGGATGCTGCTGGAGGATACCCT20.1607717041800643No Hit
CAAGAATAACATATCAACAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTG20.1607717041800643No Hit
TACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAAC20.1607717041800643No Hit
TGGCACAAGAGTTGGCCAGGATGGGCTCCAGGGGGGCCGTGGGAGAGACC20.1607717041800643No Hit
GCTTTTGGATGGAGGGTTCTGTGTGTATCTGTCAAGTCCATCCAAGTAAG20.1607717041800643No Hit
GTACACTCTTAAGAGCCTTTGCATATCAGTGGTTTCAGGGAACATCTCTC20.1607717041800643No Hit
GTCTCTCTCCCCAGCTCTGTGTCAAATAAATAAATGGCATCTTTAAAAAA20.1607717041800643No Hit
CAGTACAATTATCCAACATTTATAAATATTTACTGCCCCAGCATGGAGCA20.1607717041800643No Hit
GAAACAGACATCTGACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGAT20.1607717041800643No Hit
GTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCATTAGTTTGAT20.1607717041800643No Hit
CTTCTCTAGCATATGCTTGTGCACTCTCTTTCTGTCTCTACAATAAATAC20.1607717041800643No Hit
GCTGACAGAGTCTCAGCCAGATTCAAAGAAAGAGGACTCATCATCAGAAA20.1607717041800643No Hit
CATCTCAAATAAGCAACCTAACTTGATACCTCAAGGAAACAGAAAAGAAC20.1607717041800643No Hit
ATATTTAGTTGCTCCATGCTGGGTGGGTAAATATTTATAAATGTTGCATC20.1607717041800643No Hit
GTATTAAAGTTCCCTATGGTATTGGTGCTGATTGCTTCTCTTAGGTTTGT20.1607717041800643No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG20.1607717041800643No Hit
TCCCAATGCCCCTACTTAACCTCTTTTCTGCCTGTTTGCCCACCTCGGAG20.1607717041800643No Hit
GAGCCAAAGGCAGAGGCTTAACCCACTGAGCCACCCAGGCGCCCCTGGGC20.1607717041800643No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT20.1607717041800643No Hit
GACTTAGAGCAGGGGTGTGCTTTGTTCATGGCTGCAGAACTAACAGATGA20.1607717041800643No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG20.1607717041800643No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)