FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1966-lungUR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1966-lungUR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562613
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA450.00963745348.332718
GTATAGG2051.1348675E-831.8345181
CTATATA1153.1589463E-431.5241362
AGCGAAA2101.4351826E-831.076551
GCGAAAG2151.8046194E-830.3538421
TACACTA2054.0311716E-728.2948325
TTTACGC1050.007702775827.6211472
CGAAAGC2651.3730823E-724.6245122
TTAGGTA2303.1370317E-522.0668954
GTTTACG2353.7007565E-521.5993061
GTTTAGG2053.6596102E-421.2230131
GCCTTAT2404.3529883E-521.149321
ATTAGGT2806.0784714E-620.715863
ACTATAT2104.311277E-420.714028
ACCGTGC2104.311277E-420.714028
TTATACT2608.064593E-519.5207144
CCTAGAC3051.2724911E-519.0178383
GTATAAT4253.257992E-818.7678011
GTATTAG1950.006830422718.592811
ATTATTC3902.7240458E-718.5911583
Produced by FastQC (version 0.11.5)