FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1966-lungUL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1966-lungUL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823821
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC15250.1851130281942315No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG10950.13291722352307114No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC9950.12077866429721991No Hit
GTAGAGGGATGACCTCATGGTCCAGGTGACACACCTGCAGGGAGGAGACA8330.10111419835134089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTA1351.0251824E-842.9654434
CTATATA1157.1383474E-637.828272
CTACTGT4500.035.4486431
GTTACGT1051.8521432E-434.52791
GCTATAT850.00274011434.121691
AACGGCG850.002741745434.11755145
ATAGTAG1000.00607212929.0016775
GTTCTAT9800.028.8554571
GTAGCGG2654.5565685E-927.3584129
GGTAGCG2654.5565685E-927.3584128
TATACCA11550.026.99295
TATATAC1907.1502236E-626.7120673
CTACATC2456.4319465E-826.6341923
CATTACC7800.026.0271433
TCTATAC10700.025.749153
ACTATAG1701.0306718E-425.5897142
TTACGTG1450.001225846725.0014462
TATTATA2051.2893281E-524.7575262
TTCTATA11150.024.7099482
CTATACC10950.024.4991324
Produced by FastQC (version 0.11.5)