FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1966-lungMR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1966-lungMR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801167
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC8520.10634486942173105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG1457.2759576E-1250.002995
AGCGAAA6050.039.547821
CGAAAGC6750.035.4465643
GCGAAAG6850.034.9290962
GGACCGT1251.264256E-534.8020826
CGCTTAC850.002740857234.119691
GTGATCG850.00274169634.1175589
GTACCAT2252.8139766E-829.0017363
TAATACT8100.028.643694
TGCGGGC1555.4991735E-528.064444145
GTAATAT3057.0940587E-1026.1491051
TCGCCAT1409.993003E-425.89279145
AACCGCG1409.993003E-425.892797
GTCTATA2101.5538464E-524.1681141
ATAGGAG2701.6470585E-724.1681123
TATAATA6300.024.1681122
GCGGTCA1550.001808203823.3870379
ACACCGT2502.2770928E-623.2013876
GTAAGAC2603.2011812E-622.3090293
CTATATC3253.98486E-822.3090274
Produced by FastQC (version 0.11.5)