FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1966-lungLR-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1966-lungLR-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891062
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG9800.10998112364796166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGG1402.7453558E-531.0730658
GTCGAAG1000.00607420928.9998993
TATACTG3351.8189894E-1228.134235
CCGACTA1306.4825214E-427.884524
GTTACGT1450.001225560925.002721
GTATTAT11300.023.741521
CGAAAGC4303.6379788E-1223.604573
TTACGTG1550.001808372123.3870142
AGCGAAA3803.619789E-1022.8972261
CTACATC4205.2750693E-1122.4403973
GCGAAAG4256.184564E-1122.1763952
TACAGCC6650.021.8044367
GATATAC2003.102743E-421.7499263
ATACAGC8050.021.6148326
TAGTACC6400.021.524579
TATAACC4551.5279511E-1020.7142143
TTATACT3901.2190867E-820.4486474
TAACCTA4351.9626896E-919.9999315
GTTATGT8350.019.9722331
TAGAGGT2556.956584E-519.9018924
Produced by FastQC (version 0.11.5)