FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1966-lungLL-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1966-lungLL-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897553
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC12810.14272137689919148No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG9890.11018847912045306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACTA1351.9099389E-1048.3331689
GTGCGAA2400.039.2706954
GTTACGT1209.555684E-636.2539141
AAGTCGA1402.7443402E-531.0747831
ACATGGT7550.030.728375
CAATTCG950.00472712130.5262099
GACGACT950.00472712130.5262094
GATATAC1204.057801E-430.2082273
GTTGACA7700.030.1331211
GCAATAC1000.006074232528.9998993
ATCGTGC1050.007708166727.6189548
GTTATGT3357.4578566E-1125.9729541
TGTGCGA3357.4578566E-1125.9700583
AGTCGAA1701.0312017E-425.5881462
GCGAAGA4151.8189894E-1224.4577476
TATACTG2701.6485319E-724.1665845
GTATATA2151.8639023E-523.60721
TACGTGT1851.8305056E-423.5134323
CCGTAGA3402.5011104E-923.4584141
GTATTAT6500.023.4256041
Produced by FastQC (version 0.11.5)