FastQCFastQC Report
Thu 3 Dec 2020
HHV3KAFX2_n01_1915_d06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1915_d06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGAAAAGAGGAGGGATGCCTGTCTGGCTTAGTTGGTAGAGAATATG22.4691358024691357No Hit
ACTGGGATAAGATTGTAGCAAATTAAATAACCAGATATCAGAAAAACAAA11.2345679012345678No Hit
GAATTAGATGGTTCTCTTTATTTTTTCAGATAGTTCATCATTAGAGTGTT11.2345679012345678No Hit
TCTTAGAATTTATTTGGGGGGATTCCTTCACTATAGTGTCTTTCCTTAGC11.2345679012345678No Hit
GTGTGCACTTGTATATTTCATTGTCGGCAGCCGCTGATGATCACATCTGT11.2345679012345678No Hit
CTCTGGTATCCCATAGACTGCTGATTCTTAAGGGAAGAGATATATCCAGA11.2345679012345678No Hit
TCAATGTATTTAATGATTAGGAATTTCCTGCCTTTGAAGAAAGAAGCTGA11.2345679012345678No Hit
GGGAAGCAGACTCCCTGCTGAGCAGAGAGCCCAATTTGGGGCTCTATCCC11.2345679012345678No Hit
TCTTGAATCTGGACTTTGTTTTTGAGTGCAAATACCATATATTTAATTGC11.2345679012345678No Hit
GAACATAGAAACGCAGATGTGGTCAGGACAGACCTTTCCTATCCCTGTCT11.2345679012345678No Hit
TTACTTGAAGTATGAAGATATTTTTGTTGCTGTTGTTTCTGCCCTTAAAA11.2345679012345678No Hit
CTTCCTGATGAGCAAAGAGCCTGATTCAGGGCTTGATCCCAGGACTCTGG11.2345679012345678No Hit
ATCTTTGGGTGGGGAAGAATGAAGGGCTTTTCCTTTTGTTTAAATATATT11.2345679012345678No Hit
ATTTTACATGTTAAATTAAACTGATTTTCCCCCAGAGCACACATTTTGCC11.2345679012345678No Hit
AGCGAAAGCAGGCCTGAGAAATAAATCTATACCCCTAAATGCATACTTTA11.2345679012345678No Hit
GTTGCGTGAGAGATGCACATCATCTGCACCGGAGTAGCTACAGGCACGGT11.2345679012345678No Hit
ATTCCATTCGGGAGAATCAGGGGACACTCCAGGCCCTTCTCCCTACCCCC11.2345679012345678No Hit
CTCCTATGAGAATGTGGTAAGACTGTTCTCTCAGGAATCGTGGGAGACTA11.2345679012345678No Hit
ACATATAGTGCCAATTTGGATCAAAAGTCTTTAATCTCTTTCTCTCCTAA11.2345679012345678No Hit
CACCATGCCATCCTTAAAACTGGAATGAGGCCTGTTGTCATGGGAAGAGC11.2345679012345678No Hit
CCATTATAGATTAATATGGGATGAATAATTTAAACAATGGAAGAGAAAAA11.2345679012345678No Hit
GCAGAAGATGATTAAATTCCACCTCTTGTTCACCAGATCACTTTGTGTGC11.2345679012345678No Hit
ATCCCAGGACCTTGGGTTTATGAGATGCTTAACCGAATAGGCCACCCAGG11.2345679012345678No Hit
GAATTATTTCAGATTAGATTTTGGTGAACTTAGAGGTAATACATGATACA11.2345679012345678No Hit
GTTTTGAGGTAGAGTCATTCTCTGGTATCCCATAGACTGCTGATTCTTAA11.2345679012345678No Hit
AGCGAAAGCAGGACCTTAGAAATTGTCTAGTCTAAGCTCTTCATTTTACC11.2345679012345678No Hit
AAATGCATACTTTAGAAAAGAGGAGGGATGCCTGTCTGGCTTAGTTGGTA11.2345679012345678No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.2345679012345678No Hit
GGGCTGATGATGTTCACCATGCAGGATTAATTGACAAAGAAGAAGTGGTC11.2345679012345678No Hit
GTATAAAGGTCCTCTAGCAGAACTGCTATAGCCTCATTTGTCGGGAGCAG11.2345679012345678No Hit
AAAGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCTTCCCGCCGAGCAGGGA11.2345679012345678No Hit
ATATAAGGGAAGCCATTTTATGTTTCTAAGTCAACATGACTGTACATAAA11.2345679012345678No Hit
GATCAGTACCCCATAAACTATGACATTGATAAAGAATTTGAAGGAGACAT11.2345679012345678No Hit
GTTTTATGACCGTAACTAAGGCTTTCCTACATGAGAGTCGTGCCCGTACA11.2345679012345678No Hit
AGTAGAAACAAGGAAAAGTAAAGCAATTTTTTTAAAGTTCAGAACAACTC11.2345679012345678No Hit
AGGTTACCCAAGTTGAGTGATCTTGAGCGCTCTCCCAAAACAGTATTCTA11.2345679012345678No Hit
GCAGGCAGAGTGGCAGGCAGAGGCAGAGAGAGAAGCAGGCTCCCCACTAA11.2345679012345678No Hit
AACTTAGAGCCAGGAGGCTTGGGTTCTAATCGCAACCCCGCCACTAACCC11.2345679012345678No Hit
GGCTATGTATAAATCAGGGGCATATGGAAAATTTCTGTACTATATGCTAC11.2345679012345678No Hit
CTTTTGTAGCAGTGCCTTTTGTTTCTTCGATTTCCTCATTTCCTACATCC11.2345679012345678No Hit
CCTCACTATGGATGCAGCATGGAAAACTGATGTGAGGGAGGTAAGAGTGG11.2345679012345678No Hit
GTGTGAACACAGGAGGAGGAGATGTTTCTCTTCCAGATGCTTGGTCTGGA11.2345679012345678No Hit
GCATTACATCCAGTGAACAAATTATATTGCCTTACACCCAGGAAACTGCA11.2345679012345678No Hit
GTCAAGAATAGATCTGAGTTTTCCAACTTGGATGCTGCTGAGAGGAGTTG11.2345679012345678No Hit
TGCTAAGAGTTCCAAAAGCACCTCAGTATACTAACTGGGGGTTATTTTTT11.2345679012345678No Hit
AGCTGTACCTCAGGAAAAATAAAACCTACAACCTACCGTTTCTGAATCAT11.2345679012345678No Hit
CTTATTATCTTTCTCAAAACCTAAAAGTGAATTATATAAAAACACTTTCG11.2345679012345678No Hit
GTATTATTCAGTCGTGTGTGTGTGTGTATAAAAATATGTTTGGCCATAGA11.2345679012345678No Hit
GATCAAATACAGAGACTGCAATATCTGCTAAAATGAAGAAGAAACTTGGT11.2345679012345678No Hit
AGGTGGGACAGAGCAGCACCAGACTCATTTGCTCTTCAGGTAGATCTTGG11.2345679012345678No Hit
CATGATGAGCAGGGAGCCCAATGCAGGGCTCGATCCCAGGACCTTGGGTT11.2345679012345678No Hit
GAGGGGACTGCCTCAGAAGGTTATTAGTAGAAACAAGAACGAAAGGTAGC11.2345679012345678No Hit
TCTTTGTAGACAGTCTCAAAACTTCTATTTTAAAATCCTAATATTTATAA11.2345679012345678No Hit
ATAAAAGACTGAGCCACCCAGGCGCCCATGAACAGGTGTTTGTTTTTGTT11.2345679012345678No Hit
AGCAGGCTCCCTGCAGAGCAGAGAGAGCCCCATGCGGATGCGGGGCTTGA11.2345679012345678No Hit
TTATATTGCATTACATCCAGTGAACAAATTATATTGCCTTACACCCAGGA11.2345679012345678No Hit
TTCTGCGACCCACCAAGGCCTGGTTTCCCCTGCCTCTGCTCCCGATTCAC11.2345679012345678No Hit
GTGCACAAGCATATGCAAGAGAAGAGAGGGTCAGACGGAGATAGGTTATC11.2345679012345678No Hit
AAGAAATACAATCCCAGAAACACATTTTTAAATGTGCAATTAAAAAAGGA11.2345679012345678No Hit
TAACAACAGCCACCCAGACACTCCTGGAGATAATTTTTTTTTTAAAGTAA11.2345679012345678No Hit
TAGCAGGGGAGCCTGATGGATGTGGGGCTCCATCCCAGAACCCTGAGATC11.2345679012345678No Hit
CTCCCAAGCATCATCACCGAGGAGCAAGGCTGCTGATGGCTGCACGGGAA11.2345679012345678No Hit
GGGAGTGGGAGGCAGAGGGAGAAGCAGGCTCCATGATGAGCAGGGAGCCC11.2345679012345678No Hit
ATATTAATTTGCTAGGGCTACCATAACAAGATACCACAGACGGGGTGACT11.2345679012345678No Hit
GTAGTATCCATTTCACATGGATACTTCATCAGAGTGTTTCCTCTTGAAAA11.2345679012345678No Hit
AGCGAAAGCAGGGGGGAGGGATAGAGGGAGAGCAGACTCCCCGCTGAGCA11.2345679012345678No Hit
GGATGTCATCGGTTTCCCTTGCCCTTTCACGTAAGATTTGAGCCACAGGG11.2345679012345678No Hit
GAAGAGACTGTTCCGGGAATAGAAGAAAAGTGTTTAAAATATAGATGTTA11.2345679012345678No Hit
ATTACAAACTAACTGACCCACCAGGGTGGGAAAACAAAACAAAACAAAAA11.2345679012345678No Hit
CCCTAAATGCATACTTTAGAAAAGAGGAGGGATGCCTGTCTGGCTTAGTT11.2345679012345678No Hit
GGAGAGGGACAAGGAGACTCCCCACCAAGTGTGGAGCCAAATGTGAGGCT11.2345679012345678No Hit
CAGGCAGACCACGTACAAATGAGCCGCACATGGTGTGTCCTGCCTGCTGC11.2345679012345678No Hit
CTTCATACACATTGTCATATTTAGTCTCCCACGATTCCTGAGAGAACAGT11.2345679012345678No Hit
TCTTTATTCTAAGGCCCCCAACTGCATTCTCCTGTGCCTCCCTTTCATCA11.2345679012345678No Hit
CAGGCACTCCAGACTTTTCTTGTTTCTTGAGGAAGACCTGCATTGCTATA11.2345679012345678No Hit
CCTAACAACTGAGCCACCCAGGTGCCCTGGACCATTGTATTTTTGTCCTT11.2345679012345678No Hit
GCCTTTTACTCTAAGCTCTGGTGTGGCCGATTTTCCTGAAGGTTGTCTGG11.2345679012345678No Hit
ACATATATCACTGGGTTAATCTTCTAAACAAAATCAACAAGGAAACAATG11.2345679012345678No Hit
GATGAGCAGGGAGCCCAATGCAGGGCTCGATCCCAGGACCTTGGGTTTAT11.2345679012345678No Hit
CACCCCCAACCTACTAAAGGGTGGGTGGAATGGGTTTTCCCCCCTCCTCC11.2345679012345678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGGT50.0145.01
ATCCCAT50.0145.08
TCCCATA50.0145.09
TGGTATC50.0145.04
TCTGGTA50.0145.02
GTATCCC50.0145.06
CTGGTAT50.0145.03
TATCCCA50.0145.07
TTCAGCA50.0145.0145
GGTATCC50.0145.05
Produced by FastQC (version 0.11.5)