FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1915-d08-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1915-d08-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529231
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTAACACAGTGCGGCCTCCCTACAGGGCTTCATGGCCACAGTAACCA9270.17515980734310727No Hit
CACCAGGGATGCTCCAATAGGACTCAACACATCTGGAAGTAATCCAAAAG5830.11015983568611815No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTAAC4700.043.1947822
GCTATAC700.001055793541.4317323
CGAAAGC2450.041.431732
GTGCTAA6550.035.425891
GCGAAAG3000.033.839111
GTCTTAT1301.6513835E-533.467251
GTACCGC2051.1355951E-831.8316976
ACAGTGC7300.031.7802419
GCTAACA6400.031.721173
GTTATAG2003.244586E-729.0049511
TAATACG1606.81862E-527.1895754
TGTACCG2756.7448127E-926.3656465
TAACACA8550.026.2885535
CTAACAC7750.026.1955454
TATACTG5000.026.101995
CTATATA1409.983912E-425.894832
TACGTTT1409.983912E-425.894835
AGCGAAA2559.452924E-825.5926061
TATAGCC2559.4614734E-825.5901853
TCAATTG1450.00122513525.0019073
Produced by FastQC (version 0.11.5)