FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1915-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1915-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821716
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG9680.11780225771434412No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9110.1108655545224871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1550.070.164531
GTGATCG1258.913048E-1152.202419
TTACGTG2250.048.3326262
CGTGTTG3000.041.0827335
TATACTG3750.038.66615
TACGTGT3200.033.983883
ACGTATG1301.6545902E-533.4610442
CAGGTCG4050.032.221757
CGTATGC1204.0577722E-430.207893
AGGTCGG4700.027.7672398
ACGTGTT4150.026.2044334
GTTGACA7600.025.7577671
TTAGAGT4450.024.4378434
GTATATC2701.647386E-724.1677841
TAGCATG11200.023.950542
GTTATGT5450.023.946061
ACATGGT8300.023.583995
TGTAGAC1550.001808370623.3867533
GCATGTT11500.023.3257454
CTATAAC2252.6510992E-522.5552253
Produced by FastQC (version 0.11.5)