FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1915-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1915-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891134
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG406.655793E-572.515371
GGACCGT2450.059.1895836
GTCGTAC701.6937076E-551.79671
CCGTGCG3300.043.938859
GACCGTG3600.040.2795377
AGCGAAA2800.038.8475231
GTGATCG750.001483389538.666199
GCGAAAG3100.035.0861172
TCGTACT1458.686402E-735.005462
GCCGTCG900.003627244232.223638
AGGACCG5300.031.4654055
TAGGACT4050.030.4351394
ACGGCTA2054.0347913E-728.295515
GTATAAG4900.028.1182061
ACCGTGT3650.027.8094358
ACCGTGC5900.027.035088
TTACGGA1658.411845E-526.3662684
ATAGCAC7200.026.1831683
TTACACT5050.025.8441644
ATGAGCG2001.0644746E-525.3775335
Produced by FastQC (version 0.11.5)