FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1915-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1915-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006418
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4050.059.0794371
TAGCGGT2250.048.3329164
CGGTATC2050.045.9752167
TATAGCG2150.043.8368342
GCGGTAT2250.041.8885276
AGCGGTA2300.040.9779135
GCGAAAG6150.038.9021072
CGAAAGC7200.034.235823
ATAGCGG2900.032.499723
CTACGGT950.004727507530.5260564
GTATCGG2003.2546268E-728.99975145
GTATATA5700.024.168861
TATACTA2151.8658762E-523.6044485
CCCTTAT5300.023.256831
GTATCCC6200.022.2175529
TTATACT11750.022.2125764
GAATAAC3304.690446E-821.969514
TAGGACT6050.021.5700654
TATACTG13250.021.339445
GTCTACG1700.003091179521.3254661
Produced by FastQC (version 0.11.5)