FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1914-d12-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1914-d12-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579351
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC14360.2478635576705659No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT7430.1282469521930574No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGG606.802138E-660.416458
AGTACCG502.0087135E-458.00485
GTGATCG657.334148E-444.6152239
GTTACGT750.001482267638.6698651
GTTATAC750.001482267638.6698653
TAGTACT800.002035082636.2534
AGTCGGT1001.3907562E-436.249879
CACCGAA1051.8518558E-434.5266651
TATGTAC1551.462653E-632.7446443
TATAGAC950.00472375930.5288412
ACTTCGG1050.007705954427.6189488
GTATTAT3204.1836756E-1127.1897491
ATTATAC2156.1126957E-726.9789773
CGGATTT1358.0746826E-426.8540745
GTCCTAT8750.026.5164781
TACTGAA5250.026.2380037
ATATAGT2001.0637774E-525.37711
TCTATAC10300.025.3419043
ACCGAAG1751.2541296E-424.85922
TCAATAC1801.518356E-424.1686673
Produced by FastQC (version 0.11.5)