FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1914-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1914-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871266
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1405.456968E-1251.7852449
GCGAAAG4500.040.286661
GTAATAT3450.039.936341
AGCGAAA3450.039.936341
CGAAAGC4900.035.5159952
TATACTG6000.035.045375
GCTATAT1652.381863E-630.7643571
GTTATGT4300.030.355531
GTATATC1204.0523824E-430.2149941
GCACCGT5400.028.1974266
CCTATAC1555.493514E-528.0690923
GCTACTA1100.009671343526.3633989
GTACAAG1958.7378285E-626.0313781
ACCGTGT6250.025.5197688
AGCACCG5750.025.2200575
TAGGACT5100.024.1692224
TAGGTCC1500.001493224.169224
TAGCACC9350.024.0399744
ATACTGT5800.023.752516
ATATACC2451.9098552E-623.6759724
Produced by FastQC (version 0.11.5)