FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1914-d04-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1914-d04-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771216
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG1105.2628657E-639.5453155
GTACTAG1752.4265319E-937.2881
GTGATCG1001.3911832E-436.249879
GCGAAAG3800.028.6201731
TAGGGTA1907.1528575E-626.710435
CGAAAGC4500.024.166582
AGCGAAA3652.1827873E-1023.8370761
CCTACAC3503.4979166E-922.7856333
ACACTGG10300.022.524196
TACTAGA2755.208276E-621.0908362
GTACCGC2104.3130034E-420.7142126
ACACCGT3508.729876E-820.7142126
TAGCACT4952.1827873E-1120.5049764
ACCGTGC2505.972394E-520.2999278
CTAGGAC5453.6379788E-1219.9540583
GTATAAG3651.3582576E-719.8642311
GTCCTAT7050.019.5402031
CTACACT3751.8068931E-719.3332634
GGTAGTG4553.428795E-919.1208117
TCCTAGC3802.0757761E-719.0801161
Produced by FastQC (version 0.11.5)