FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1914-d02-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1914-d02-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907887
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCC450.0096390448.3330969
GTAGCCG2207.485014E-726.3635069
TATACTG5350.024.395095
GGTTATA2502.2756594E-623.203721
TCGCCAT2552.7067454E-622.744986145
GTACCGC1600.002173666822.6586346
GTATAGT2252.6479134E-522.559171
TATACAG7400.022.5361565
GCGAAAG4006.89397E-1021.7522892
TTCGACA2003.1005265E-421.7522892
ATACAGT7050.021.5980176
CTAGGAC7150.021.2959483
GTTATAC2908.240613E-620.0021063
AGCGAAA4001.6279955E-819.9406971
TATAATA6200.019.8811242
GTATTAT9000.019.3364321
GTGTCGG3451.7417333E-618.91295145
GTCCTAT10750.018.886751
TAGGACT9050.018.4273534
GTACTGG4755.840775E-918.3187261
Produced by FastQC (version 0.11.5)