FastQCFastQC Report
Thu 10 Dec 2020
HHV3KAFX2_n01_1913-d06-rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV3KAFX2_n01_1913-d06-rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046999
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC33640.32129925625525907No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG25950.24785123959048672No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT25230.24097444219144432No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC21710.2073545437961259No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG20250.19340992684806765No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCAGACCATTGACAAGG20020.19121317212337355No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG17290.16513864865200445No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT16960.16198678317744336No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC16110.15386834180357384No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA15710.15004789880410582No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA15610.14909278805423884No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG15330.14641847795461121No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC15210.14527234505477082No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT15020.1434576346300235No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA14670.140114747005489No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG12780.1220631538330027No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG12760.1218721316830293No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC12750.1217766206080426No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC12580.12015293233326871No Hit
CCCTTAGGTGACTCTCCAATTGGCCATGTCTCCGATCTGTTTTCAAAGAA12060.1151863564339603No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA11720.1119389798844125No Hit
CTATAGGACTTGCAAGCTAGTTGGAATCAACATGAGCAAAAAGAAGTCTT11630.11107938020953219No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG11500.10983773623470511No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC11410.1089781365598248No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC10750.1026744056107026No Hit
CCATAGAGGTATTCAGATCGAACGGTCTAACAGCCAATGAGTCAGGAAGG10700.10219685023576909No Hit
TTTCTAAACCATTCAGGTTGGTTCCTTGTGATGTATGTTATCATTGCTAG10630.10152827271086219No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA10540.1006686730359819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTG2100.082.860715
GCAGGTA2200.079.094322
AGTAGAA3000.067.672811
AACACTG5300.065.663215
AGGTACT3450.058.843124
GCGAAAG1101.6752892E-952.729552
TATAGCG9150.050.712572
GACAGTA4250.049.470367
TATACTA5350.048.787152
GCGGTAT9800.048.0888146
TATACTG5650.047.4799235
ATATCAC1402.728484E-1046.609153
GTATCCC10200.046.2007649
CCCTTAT5650.046.1988871
CACTGTG7800.044.6151777
GTACTGA5050.044.5068746
TTATACT6100.043.977314
CGGTATC10950.043.036337
CTACTAG5900.043.0103346
TTTGGAC4800.042.293493
Produced by FastQC (version 0.11.5)